PRKD3 antibody (AA 352-384)

Catalog No. ABIN2795130

This anti-PRKD3 antibody is a Rabbit Polyclonal antibody detecting PRKD3 in WB, IHC and IF. Suitable for Human.

Quick Overview for PRKD3 antibody (AA 352-384) (ABIN2795130)

Target: PRKD3 (Protein Kinase D3 (PRKD3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKD3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-PRKD3 Antibody

Binding Specificity: AA 352-384

Purpose: Rabbit Anti-Human PKC nu Antibody

Immunogen: This PKC-nu antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 352-384 amino acids from human PKC-nu.

Isotype: Ig Fraction

Application Details

Application Notes: Western Blot, Immunohistochemistry
Recommended Dilutions
WB: 1:1000, IHC: 1:50-100Western blot analysis of anti-PKCnu Pab antibodies. Lower panel, indirect immunofluorescence analysis of GFP-PKD3 fusion protein expression in Panc-1 cells by using 102-17077 and 102-17078 antibodies. Data courtesy of Dr. Osvaldo Rey, University of California Los Angeles.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry, clinical relevance has not been evaluated. BC = breast carcinoma, HC = hepatocarcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.200 mg/mL

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C (short-term), -20°C (long-term)

Target Details for PRKD3

Target: PRKD3 (Protein Kinase D3 (PRKD3))

Alternative Name: PKC nu

Background:

Target Description: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. PKC nu is one of the PKC family members. This kinase can be activated rapidly by the agonists of G protein-coupled receptors. It resides in both cytoplasm and nucleus, and its nuclear accumulation is found to be dramatically enhanced in response to its activation. This kinase can also be activated after B-cell antigen receptor (BCR) engagement, which requires intact phopholipase C gamma and the involvement of other PKC family members.

Gene Symbol: PRKD3

Molecular Weight: 100471 Da

Gene ID: 23683

UniProt: O94806